Two Methodological Limitations

Methodological Limitations in Determining Astrocytic Gene Expression

Traditionally, astrocytic mRNA and protein expression are studied by in situ hybridization (ISH) and immunohistochemically. This led to the concept that astrocytes lack aralar, a component of the malate-aspartate-shuttle. At least similar aralar mRNA and protein expression in astrocytes and neurons isolated by fluorescence-assisted cell sorting (FACS) reversed this opinion. Demonstration of exp...

Neuroimaging and ADHD: fMRI, PET, DTI findings, and methodological limitations.

Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder characterized by pervasive and developmentally inappropriate levels of inattention, impulsivity, and hyperactivity. There is no conclusive cause of ADHD although a number of etiologic theories have been advanced. Research across neuroanatomical, neurochemical, and genetic disciplines collectively support a physiolo...

Methodological and Conceptual Limitations in Exercise Addiction Research

The aim of this brief analytical review is to highlight and disentangle research dilemmas in the field of exercise addiction. Research examining exercise addiction is primarily based on self-reports, obtained by questionnaires (incorporating psychometrically validated instruments), and interviews, which provide a range of risk scores rather than diagnosis. Survey methodology indicates that the ...

Birth Outcomes and Natural Gas Development: Methodological Limitations

Putting the methodological brakes on claims to measure national happiness through Twitter: Methodological limitations in social media analytics

With the rapid global proliferation of social media, there has been growing interest in using this existing source of easily accessible 'big data' to develop social science knowledge. However, amidst the big data gold rush, it is important that long-established principles of good social research are not ignored. This article critically evaluates Mitchell et al.'s (2013) study, 'The Geography of...